【草魚】長見識了！抗菌肽原來是這樣影響魚類生長和免疫功能的！

欄目：水產案例發布時間：2021-06-03

圣西馬攜手四川農業大學發表權威期刊論文

養魚的朋友相信深有體會，在高密度飼養環境下，魚類腸炎的發生率大量增加。腸炎的發生往往會引起腸道免疫功能的紊亂，降低魚的生長。但是，抗菌肽對于魚類腸道健康的作用，目前未見深入的研究。

圣西馬攜手四川農業大學開展了《蝎源性抗菌肽對生長中期草魚的生產性能及腸道健康的調控作用》的研究，該研究可以為揭示抗菌肽調控魚腸道健康的作用機制提供部分理論依據，此外，還確定了生長中期草魚飼料中添加抗菌肽IsCT的最適添加量，在草魚飼料配制時可供參考。該論文發表在ELSEVIER（愛思唯爾）旗下數據庫ScienceDirect《水產養殖》（Aquaculture）期刊上，歡迎朋友們前往閱覽。

ScienceDirect，簡稱SD，是全球最權威的多學科全文期刊數據庫，對全球的學術研究做出了巨大貢獻，每年下載量高達10億多篇，在所有學術類數據庫中下載量最大。

論文刊載截圖

HIGHLIGHTS ● 要點概覽

● Fish fed antimicrobial peptide (AMP) promoted the growth of fish.

抗菌肽可以提高草魚的生長速度。

● Fish intestinal immune function were improved with AMP supplemented in diets.

日糧中添加抗菌肽，可以改善草魚的腸道免疫功能。

● AMP modulated molecules of JAKs/STATs signalling pathways of fish intestines.

抗菌肽可能通過 JAKs/STATs 信號通路調節草魚腸道炎癥因子。

ABSTRACT ● 摘要

This dissertationwas primarily explored the impacts of antimicrobial peptide(AMP), Isalo scorpion cytotoxic peptide (IsCT), on growth and intestinal immunefunction of fish. For this objective, 540 fish (136.88 ± 0.72 g)were randomly selected for 6 treatments for a growth trial and fed 6 levels ofAMP (0, 0.6, 1.2, 1.8, 2.4, 3.0 mg/kg diet) within 60 days. Afterthat, grass carp werechallenged by A. hydrophila for 6 days. This research revealedthat appropriate AMP supplementation:

(1) increased fish growth performance andenhanced the enteritis resistance.Notably, quadratic regression analysis of PWG and enteritis morbidity, theoptimal dose of AMP (IsCT) for on-growing grass carp (136.88–507.78 g)were calculated to be 1.52 and 2.00 mg/kg diet, respectively.

(2) improvedthe intestinal immunity of fish partially resulted from increased above immunesubstances activities and expression [e.g., acidphosphatase (ACP) β-defensin-1];

(3) attenuated the fishintestinal inflammatory response by up-regulating the studied anti-inflammatorycytokines gene expression and down-regulate the main pro-inflammatorycytokines, which might be partly regulated to janus kinases(JAKs) / signal transducers and activators of transcription (STATs) signallingpathways molecules. However, AMP supplementation did not alter STAT2 andSTAT3a gene expression in fish intestines. In conclusion, this study providedevidences that AMP enhanced growth performance and improved intestinal immunefunction and systematically researched its related pathway (JAKs/STATssignalling pathway).

本論文主要探討了蝎源性抗菌肽（IsCT）對魚類生長性能和腸道免疫功能的影響。試驗選擇540尾平均體重為136.88±0.72g的健康草魚，隨機平均分成6個處理組，分別飼喂含不同濃度IsCT(0、0.6、1.2、1.8、2.4、3.0 mg /kg)的試驗飼糧60天。在生長試驗期之后，開展為期6天的嗜水氣單胞桿菌攻毒試驗。
注：本研究使用的蝎源性抗菌肽（IsCT）由深圳市圣西馬生物技術有限公司提供。

草魚腸道切片